mouse anti rat ed1 primary antibody  (Bio-Rad)

Journal: Biomaterials Research

Article Title: Improvement of Charcot-Marie-Tooth Phenotype with a Nanocomplex Treatment in Two Transgenic Models of CMT1A

doi: 10.34133/bmr.0009

Figure Lengend Snippet: Investigation of the anti-inflammatory effect of NanoCur in CMT1A. (A) Anti-CD68 [ED1] staining of sciatic nerves using 3,3-diaminobenzidine staining (IHC) for macrophage detection in sciatic nerve sections of WT-NT, CMT1A-NT, and CMT1A-NanoCur rats, in addition to the quantification of the percentage area of macrophages present in the nerve sections. Representative figures along with barograms of the quantification of the average protein expression level of (B) Macrophage Migration Inhibition Factor MIF and (C) myeloperoxidase with respect to the total protein. (D) Stain-free gel of the representative figure for MIF (Gel 1) and myeloperoxidase (Gel 2) (E) Representative figure of the fluorescent staining of mouse sciatic nerves of WT, Tg +/- , Tg +/- -T, Tg +/+ , and Tg +/+ -T with the macrophage marker F4/80. Results are represented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA test followed by post hoc Tukey test; *: P < 0.05.

Article Snippet: The sciatic nerves of rats were stained with rat anti-CD68 (ED1) primary antibody (Biorad, MCA341, 1:1,000) using the avidin-biotin 3,3-diaminobenzidine peroxidase kit (Vectastain; Vector Laboratories) in accordance with the supplier’s protocol and counterstained with Mayer’s hematoxylin, then observed using a Nikon optical microscope.

Techniques: Staining, Expressing, Migration, Inhibition, Marker

Journal: Molecules

Article Title: In Vitro and In Vivo Biocompatibility Studies of a Cast and Coated Titanium Alloy

doi: 10.3390/molecules25153399

Figure Lengend Snippet: Mononuclear and multinucleated cells stained with ED1 (arrow) are visible on the surface of the implant (I) ( a , 14 days, coated and b , 30 days, uncoated). ( c ) Comparing the number of positive cells among the different time points, no statistically significant difference is evident between the two implant types. ctd—coated and unctd—uncoated.

Article Snippet: For immunohistochemistry grindings were deacrylated in 2-Methoxyethyl acetate for 15 min, hydrated in acetone–acetone/washing buffer–washing buffer (TBS-X: Tris-NaCl, pH 7.4: 0,05 M Tris, Roth, 4855; 0,15 M NaCl, Sigma-Aldrich, 31434, 0,025% TritonX-100, Sigma-Aldrich, X100), blocked 10 min in 3% H 2 O 2 in TBS-X, rinsed in TBS-X, incubated 60 min at room temperature in primary antibody against rat CD68 (ED1, Sigma-Aldrich, MAB 1435) 1:5000 in Antibody Diluent (Dako, S302283), 45 min incubation in secondary antibody (biotinylated rabbit-anti-mouse (Dako, E0354) 1:400 in TBS, 1%BSA (Sigma-Aldrich, A3912), 12.5% Rat Serum (Sigma-Aldrich, R9759), rinsed in TBS-X, incubated in StreptABComplex/HRP (Dako, K0377), after rinsing incubated in NovaRed (Vector, USA, SK-4800), counterstained with Shandon Instant Hematoxylin (Thermo Scientific, Kalamazoo, MI, USA, 6765014), blued in tap water, dehydrated with ethanol and xylene and covered with DePex (Serva, 18243).

Techniques: Staining